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Image Search Results
Journal: Frontiers in Genetics
Article Title: The role of microRNAs in defining LSECs cellular identity and in regulating F8 gene expression
doi: 10.3389/fgene.2024.1302685
Figure Lengend Snippet: microRNAs targeting F8 gene. (A) Relative expression of F8 gene (Probe ID: ILMN_1675083) in the used ECs and LSECs samples. (B) Left panel: Unsupervised, 3D-PCA displaying different endothelial cells for 25 expressed microRNAs that bind to F8 gene according to IPA. Middle panel: heat map showing the expression of these 25 microRNAs in three LSEC samples. Right panel: Correlation between F8 expression in different endothelial cells and PCA-1, X-axis represents F8 expression and y-axis represents PCA-1. (C) A depiction of the 1808-base pair long 3′ mRNA sequence of the F8 gene along with the 25 expressed microRNAs known to bind to this sequence. (D) Box plots illustrating the expression levels of four microRNAs in different endothelial cells, potentially binding to the F8 gene, as well as the transcription factors binding to the F8 gene promoter. (E) Top Panel: An IPA-generated figure showing the relationships between potentially F8 -binding microRNAs and transcription factors identified using TRANSFAC, which bind to the F8 promoter. Sequence logos of individual transcription factors are displayed on the right. Bottom Panel: Representation of the F8 promoter, covering 1,200 base pairs with 1 kb before the transcription start site and 200 base pairs after the transcription start site. The numbers represent the transcription factors binding sites identified using TRANSFAC (shown and numbered in top panel above).
Article Snippet: In this study,
Techniques: Expressing, Sequencing, Binding Assay, Generated